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Frequently Asked Questions For depositors

What are the benefits of depositing my cells with EBiSC?

The benefits for individual researchers:

a) EBiSC supports dissemination of your lines. The Bank saves you time by responding to researchers who request samples of your cell lines. Once the lines have been deposited you can simply direct interested researchers to EBiSC who will manage requests for those lines on your behalf.

b) EBiSC provides backup storage for your lines. If your cryo-freezers fail or your locally held stocks become contaminated, you will have opportunities to request samples of your lines to be returned to your lab, with only the shipping to pay.

c) EBiSC increases recognition and discoverability of your research. Your deposition is recognised in the EBiSC official cell line name and clearly displayed in the EBiSC catalogue.

The benefits for a consortium creating cell lines:

EBiSC can provide a consortium with a future proofed strategy for the maintenance and distribution of the cell lines. This will not only save your consortium money, but will be seen positively by prospective research funding councils.

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If I deposit cells with EBiSC, how does this affect my ownership of them?

Cell lines deposited with EBiSC remain the property of the Depositor. This is relevant for Users who wish to directly exploit the cells on a commercial basis; it is the responsibility of the User to contact the technology transfer office of the Depositor to determine whether express permission or agreement in support of the commercial activity is required. These requirements are clearly highlighted in the agreements that purchasers have to sign before lines are distributed to them.

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If I change my mind, can I have my cell lines and associated information returned to me?

Any original iPSC material that has been deposited with EBiSC, but not wholly consumed in the qualification process, may be returned to the Depositor upon request. You may also request the return of any unconsumed primary tissue samples from which the iPSC lines were made if EBiSC partners have been involved in the reprogramming process.

However, once the iPSC lines are qualified and made available in the EBiSC catalogue they, and any original data required to describe the cell lines, cannot be returned to the Depositor.

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Once I have deposited cells with EBiSC, am I prevented from sharing these lines directly with others?

No. Your agreement to let EBiSC bank and distribute your cell lines does not limit your own ability to share the cell lines with others. EBiSC does encourage Depositors to support the mutual aim of the EBiSC project, which is to act as a hub for dissemination of quality-controlled cells to researchers worldwide.

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Is deposition of cells with EBiSC a free process?

Yes. If EBiSC agrees to bank your cells then you will not be asked to pay for this process.

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Do I have to be an EBiSC project partner to deposit cells with EBiSC?

No. Anyone can apply to have lines deposited with EBiSC.

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What information will I need to provide before lines can be deposited?

EBiSC must be able to trace the terms of consent given by the human donor who supplied originating biomaterial for derivation of the iPS cell line. You will need to provide an anonymised copy of the consent form together with the accompanying patient information sheet. The consent must:

Where the consent paperwork is acceptable, data such as the age and sex of the donor and the passage number, reprogramming technology and media requirements will be asked for and uploaded to the hPSCreg website.

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What is hPSCreg?

hPSCreg.eu is the human Pluripotent Stem Cell Registry, a database for pluripotent stem cells (embryonic and induced pluripotent stem cells). hPSCreg collects and publicly releases information about stem cell lines from the groups who derive them. The database is hosted and maintained by the Charité Medical University, Berlin. All EBiSC cell lines are registered on hPSCreg. For more information please refer to the hPSCreg about pages.

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Can I assume that EBiSC will accept all and any of the lines I wish to deposit?

No, deposit is subject to EBiSC approval of each line you aim to deposit into the Bank. The EBiSC catalogue will be selectively developed according to the needs of the research community and resources of the Bank to characterise and curate them.

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What does the Bank do with cell lines that I deposit?

After approval of the consent obtained at tissue procurement, completion of an EBiSC Material Deposit Agreement (EMDA), and collection of cell line characterisation into the hPSCreg portal, a number of vials (3-5) can be shipped to our Central Facility. The Bank may then further characterise and expand the cell line, and cryopreserve 30-50 vials for storage and distribution. A number of vials of cells will be transferred to the facilities of the European Collection of Authenticated Cell Cultures (ECACC) in the UK for distribution, and to the ‘mirror bank’ established by the Fraunhofer Institute for Biomedical Engineering (IBMT) in Sulzbach, Germany, for secure storage.

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If more than one entity has been involved in the generation of cell lines that are ultimately deposited in the Bank, does it matter which party deposits them? Is there any financial benefit to being the designated Depositor?

It is the responsibility of the entities involved in cell line generation and manipulation to decide who is the appropriate party to deposit the lines with EBiSC. Depending on investments of time, expertise and funds the Depositor may be the organisation that carried out the reprogramming to create the iPSC line, the party conducting further work such as gene editing to generate a variant of the line, or perhaps a third party who has commissioned one of the others to undertake such work.

Deposition of cell lines into the EBiSC catalogue is a voluntary contribution which does not carry any explicit financial or commercial advantage. If a User of an EBiSC line should foresee commercial activity involving a banked cell line, i.e. activities that fall outside the scope of ‘research use’ as defined in the EBiSC Access & Use Agreement, then the User would have all the necessary information to contact a Depositor to obtain permission or licensing arrangements in order to support any such activity.

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As the Depositor, can I restrict or control the purposes for which my cell lines are used?

No. If however you have an obligation to a third party that cannot be waived, or one that requires that it be passed on to the end user of the cell line, then such Third Party Obligation must be identified clearly in the EBiSC Access & Use Agreement. ‘TPO’s would include, for example, ‘reach through’ intellectual property rights of a patent holder, or the limitations imposed by a tissue donor regarding use of their samples in a specific field of research.

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