WTSIi204-B

HPSI0414i-oulr_4

iPSC line

This (hPSCreg) cell line is not part of EBiSC.

General#

Cell Line

hPSCreg Name WTSIi204-B
Alternative name(s)
HPSI0414i-oulr_4
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg WTSIi204-B
BioSamples SAMEA3847991
ECACC 77650356
HipSci HPSI0414i-oulr_4
Cellosaurus CVCL_AJ91
CLO CLO_0102114
Wikidata Q54890727

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 15-19
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
monogenic diabetes
Synonyms
  • monogenic diabetes mellitus

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2464827
HipSci HPSI-oulr

hIPSC Derivation#

General

Source cell type
mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
  • mononuclear leukocyte
Source cell origin
blood
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
  • portion of blood
  • vertebrate blood
Age of donor (at collection) 15-19
Collected in 2015
Source cell line vendor University of Exeter Medical School

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
33.959 1.338

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-oulr_4
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Raw sequencing reads
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Splice-aware STAR alignment
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Array signal intensity data
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Text file with probe intensities