WTSIi198-A
Donor information | |
|---|---|
| Gender: | Female |
| Age: | 45-49 |
| Ethnicity: | Mixed(English/Cuban) |
Disease status | |
| Disease: | monogenic diabetes |
General information | |
| Depositor: | Wellcome Trust Sanger Institute |
| Cell line name: | WTSIi198-A |
| Cell line alternative names: | HPSI0414i-fuai_1 |
| Biosamples ID: | SAMEA2796155 |
| hPSCreg name: | WTSIi198-A |
| Derivation country: | United Kingdom |
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Derivation
Derivation
Primary cell type: Fibroblast Primary cell line vendor: University of Exeter Medical School Location of primary tissue procurement: Zone of skin Tissue collection year: 2014 Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: - Passage method: EDTA Matrix: Vitronectin CO2 concentration: 5% O2 concentration: 20 Temperature: 37C -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles SSEA-4 Yes SSEA-1 No TRA 1-60 Yes POU5F1 (OCT-4) Yes PluriTest: Pluripotency score: 23.021
Novelty score: 1.398
Report:
HPSI-fuai.pluritest.pluripoten_ePzZtmH.png - pluripotency image
HPSI-fuai.pluritest.novelty_sc_mjKXJTP.png - novelty image
The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Genotyping
Genome-wide analysis: RNA-seq
https://ega-archive.org/datasets/EGAD00001003530cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-fuai_1Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.