WTSIi191-B

HPSI0215i-oilg_1

iPSC line

This (hPSCreg) cell line is not part of EBiSC.

General#

Cell Line

hPSCreg Name WTSIi191-B
Alternative name(s)
HPSI0215i-oilg_1
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg WTSIi191-B
BioSamples SAMEA3485963
ECACC 77650300
HipSci HPSI0215i-oilg_1
Cellosaurus CVCL_AX08
CLO CLO_0101197
Wikidata Q54890563

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 65-69
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3249666
HipSci HPSI-oilg

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 65-69
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
15.642 1.292

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0215i-oilg_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560639
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376185
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860775
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860679
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860871
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368786
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629709
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560623
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560541
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861063
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860487
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859911
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860583
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860007
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447887
GATK haplotype calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369078
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629699
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376044
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860967
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861255
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860391
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861159
Raw sequencing reads