WTSIi138-A

HPSI0414i-mita_2

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi138-A
Alternative name(s)
HPSI0414i-mita_2
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi138-A
BioSamples SAMEA2724743
HipSci HPSI0414i-mita_2
Cellosaurus CVCL_AF22
ECACC 77650194
CLO CLO_0101147
ECACC 66540246
Wikidata Q54890715

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 65-69
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2474448
HipSci HPSI-mita

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 65-69
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
60.825 1.764

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 18

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-mita_2
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 4
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267039
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266715
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266905
BWA alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203442
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1203466
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266809
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369113
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203394
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203426
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368821
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861346
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448104
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266958
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1243469
Raw sequencing reads