WTSIi105-D

HPSI0313i-airc_6

iPSC line

This (hPSCreg) cell line is not part of EBiSC.

General#

Cell Line

hPSCreg Name WTSIi105-D
Alternative name(s)
HPSI0313i-airc_6
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)

External Databases

hPSCreg WTSIi105-D
BioSamples SAMEA2398072
HipSci HPSI0313i-airc_6
Cellosaurus CVCL_AE69
CLO CLO_0101093
Wikidata Q54890597

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398654
HipSci HPSI-airc

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement
Serum concentration: %

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
45.478 1.319

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0313i-airc_6
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Array signal intensity data
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Genotyping array calls
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Imputed and phased genotypes