WTSIi046-A

• Derivation

  Derivation
  Primary cell type:	Fibroblast
  Primary cell line vendor:	Cambridge BioResource
  Location of primary tissue procurement:	Zone of skin
  Tissue collection year:	2014
  Reprogramming method
  Vector type:	Non-integrating
  Vector:	Sendai virus
  Gene list:	KLF4 MYC POU5F1 SOX2
  Is the reprogramming vector detectable:	Unknown
  Derivation
  Xeno free conditions:	no
  Derived under gmp:	no
  Available as clinical grade:	no

• Culture conditions

  Culture Conditions
  Surface coating:	Vitronectin
  Passage method:	Enzyme-free cell dissociation / EDTA
  CO2 concentration:	5%
  Culture medium:	TeSR™ E8™
  Passage Number banked (pre-EBiSC):	32

• Characterization

  Characterization
  Analysis of Undifferentiated Cells
  Marker expression:	Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles TRA 1-60 Yes NANOG Yes SSEA-4 Yes SSEA-1 No POU5F1 (OCT-4) Yes SOX2 Yes
  PluriTest:	Pluripotency score: 38.268 Novelty score: 1.5 Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/ Report: HPSI-wibj.pluritest.pluripoten_dcrELu6.png - pluripotency image HPSI-wibj.pluritest.novelty_sc_PDF659j.png - novelty image The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html). PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
  Microbiology / Virus Screening
  HIV 1:	Negative
  HIV 2:	Negative
  Hepatitis B:	Negative
  Hepatitis C:	Negative
  Mycoplasma:	Negative
  Sterility
  Inoculation for microbiological growth:	No Contaminants Detected
  Mycoplasma:	Not Detected
  Viability:	Viable post-cryopreservation

• Genotyping

  Genotyping
  Genotyping
  Genome-wide analysis:	Genotyping array http://www.ebi.ac.uk/ena/data/view/ERZ127647 RNA-seq http://www.ebi.ac.uk/ena/data/view/ERR914315 Exome sequencing http://www.ebi.ac.uk/ena/data/view/ERR947051 cnv http://www.hipsci.org/lines/#/lines/HPSI0214i-wibj_2 Whole genome sequencing http://www.ebi.ac.uk/ena/data/view/ERR1860424 Methylation profiling http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
  STR/Fingerprinting:	A 16 allele profile has been recorded and data is available upon request, after cell line purchase.