WTSIi046-A
Donor information | |
---|---|
Gender: | Female |
Age: | 55-59 |
Ethnicity: | White - White British |
Disease status | |
Disease: | normal |
General information | |
Depositor: | Wellcome Trust Sanger Institute |
Cell line name: | WTSIi046-A |
Cell line alternative names: | HPSI0214i-wibj_2 |
Biosamples ID: | SAMEA2627567 |
hPSCreg name: | WTSIi046-A |
Derivation country: | United Kingdom |
Related lines | |
From the same donor: | WTSIi046-B |
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Derivation
Derivation
Primary cell type: Fibroblast Primary cell line vendor: Cambridge BioResource Location of primary tissue procurement: Zone of skin Tissue collection year: 2014 Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Vitronectin Passage method: Enzyme-free cell dissociation / EDTA CO2 concentration: 5% Culture medium: TeSR™ E8™ Passage Number banked (pre-EBiSC): 32 -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles TRA 1-60 Yes NANOG Yes SSEA-4 Yes SSEA-1 No POU5F1 (OCT-4) Yes SOX2 Yes PluriTest: Pluripotency score: 38.268
Novelty score: 1.5
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Report:
HPSI-wibj.pluritest.pluripoten_dcrELu6.png - pluripotency image
HPSI-wibj.pluritest.novelty_sc_PDF659j.png - novelty image
The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Genotyping
Genome-wide analysis: Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127647RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914315Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947051cnv
http://www.hipsci.org/lines/#/lines/HPSI0214i-wibj_2Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860424Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.