WTSIi037-A

HPSI0114i-zoxy_3

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name WTSIi037-A
Alternative name(s)
HPSI0114i-zoxy_3
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi037-A
BioSamples SAMEA2536413
HipSci HPSI0114i-zoxy_3
Cellosaurus CVCL_AE41
ECACC 77650164
CLO CLO_0100998
Wikidata Q54890461

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclones

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 60-64
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2399102
HipSci HPSI-zoxy

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 60-64
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
28.014 1.243 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0114i-zoxy_3
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 0
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947038
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267046
Abundances of transcripts
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123060
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122993
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914313
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947046
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914349
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861378
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448133
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122927
mpileup variant calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127583
Genotyping array calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127348
Imputed and phased genotypes