WTSIi018-B-1

HPSI0114i-kolf_2-C1

General

Cell Line

hPSCreg name WTSIi018-B-1
Cite as:
WTSIi018-B-1 (RRID:CVCL_9S58)
Alternative name(s)
HPSI0114i-kolf_2-C1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi018-B
(HPSI0114i-kolf_2)
WTSIi018-B-21
(Kolf 2.1 J (TARDBP M337V SNV/SNV))
WTSIi018-B-22
(Kolf 2.1 J (TARDBP Q331K WT/SNV))
WTSIi018-B-23
(Kolf 2.1 J (TARDBP Q331K SNV/SNV))
WTSIi018-B-24
(Kolf 2.1 J (FUS R495X WT/SNV))
WTSIi018-B-25
(Kolf 2.1 J (FUS R495X SNV/SNV))
WTSIi018-B-26
(Kolf 2.1 J (FUS R521H WT/SNV))
WTSIi018-B-27
(Kolf 2.1 J (FUS R521H SNV/SNV))
WTSIi018-B-18
(Kolf 2.1 J (TARDBP M337V WT/SNV))
WTSIi018-A
(HPSI0114i-kolf_3)
Last update 29th July 2022
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Provider

Generator Wellcome Sanger Institute (WTSI)
Owner Wellcome Sanger Institute (WTSI)
Distributors
Derivation country United Kingdom

External Databases

HipSci HPSI0114i-kolf_2-C1
Cellosaurus CVCL_9S58
BioSamples SAMEA7996638
Wikidata Q54890432

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Non-disease associated phenotypes
  • Healthy

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398402
HipSci HPSI-kolf

Ethics

Also have a look at the ethics information for the parental line WTSIi018-B .
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Lifetech
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution can only be used for academic reserach not commercial

hIPSC Derivation

General

The source cell information can be found in the parental cell line WTSIi018-B.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Methods used
Immunostaining

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
No

Culture Conditions

Feeder cells
No
Medium TeSR™ E8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
DNMT3B
Yes
GDF3
Yes
nodal
Yes
podxl
Yes
xist
Yes
LEFTY1
Yes
foxd3
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
normal
Passage number: 30
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes

Genetic Modification