WTSIi018-B

HPSI0114i-kolf_2

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name WTSIi018-B
Alternative name(s)
HPSI0114i-kolf_2
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi018-B
BioSamples SAMEA2547615
HipSci HPSI0114i-kolf_2
Cellosaurus CVCL_AE29
ECACC 77650100
CLO CLO_0100975
Wikidata Q54890431

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclones

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 55-59
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398402
HipSci HPSI-kolf

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 55-59
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
48.039 0.876 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0114i-kolf_2
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 0
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266677
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266867
BWA alignment
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127287
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1203463
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1901046
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266771
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127522
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203391
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203423
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447925
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266990
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203439
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266946
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1243466
Raw sequencing reads