WTSIi009-A

HPSI0913i-diku_1

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi009-A
Alternative name(s)
HPSI0913i-diku_1
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi009-A
BioSamples SAMEA2593858
HipSci HPSI0913i-diku_1
Cellosaurus CVCL_AH28
ECACC 77650088, 66540079
CLO CLO_0100959
Wikidata Q54891443

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclones

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 60-64
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2398076
HipSci HPSI-diku

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 60-64
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Vitronectin
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-4
Yes
SSEA-1
No
TRA 1-60
Yes
Pluripotency Score Novelty Score Link to microarray data
28.091 1.259 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0913i-diku_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914340
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861318
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267009
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123023
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127607
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914304
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947069
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127372
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123090
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448008
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122957
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947061
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768