WTSIi001-A

HPSI1213i-pahc_4

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name WTSIi001-A
Alternative name(s)
HPSI1213i-pahc_4
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi001-A
BioSamples SAMEA2547633
HipSci HPSI1213i-pahc_4
Cellosaurus CVCL_AI21
ECACC 77650046
CLO CLO_0100946
Wikidata Q54891726

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 55-59
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398906
HipSci HPSI-pahc

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 55-59
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
28.149 1.273 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1213i-pahc_4
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860844
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860748
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860940
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122930
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR947007
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR847021
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861228
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860364
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860460
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122996
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR780167
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127561
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266994
Abundances of transcripts
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127326
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR946982
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1173376
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860652
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860556
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123063
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861036
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859884
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859980
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861132
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447942
GATK haplotype calls