|Nucleotide sequence HGSV:||NM_198578.3(LRRK2):c.6055G>A|
|Protein sequence HGSV:||NP_940980.3:p.(Gly2019Ser)|
|Depositor:||University of Oxford|
|Cell line name:||UOXFi007-A|
|Cell line alternative names:||MK002-4|
|From the same donor:||UOXFi007-B, UOXFi007-C|
|Lines from donor's relatives:|
Primary cell type: Fibroblast of dermis Location of primary tissue procurement: Zone of skin Tissue collection year: 2011 Passage number reprogrammed: 4
Vector type: Integrating Vector: Virus Virus type: Retrovirus Gene list:
Have the reprogramming vectors been silenced: Yes Merthods used: rtpcr
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no
Medium: mTeSR Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 20% Temperature: 37C
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles SSEA-1 No TRA 1-60 Yes - SSEA-4 Yes NANOG Yes - PluriTest: Pluripotency score: 21.44
Novelty score: 1.4
The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation
Passage number: 13 Cell line karyotype: 46XX Karyotyping method: Molecular karyotyping by SNP array Image / Result
Whole genome sequencing
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.