UNEWi027-A

F116

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name UNEWi027-A
Alternative name(s)
F116
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University of Newcastle (UNEW)
Owner Institute of Genetic Medicine
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg UNEWi027-A
BioSamples SAMEA4458542
Cellosaurus CVCL_IU00
ECACC 66540406
CLO CLO_0101606
Wikidata Q54991232

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 65-69

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Retinitis pigmentosa
The donor is a carrier of a disease-associated mutation and affected.
Stage
Severe
Genetic variants
PRPF31 (target)
Heterozygous
Heterozygous deletion in exon 11 of PRPF31 (PRPF31: c.1115_1125 del11)
Disease associated phenotypes
  • PRPF31
  • Retinitis Pigmentosa
  • Age of onset: 10 years old
  • Presented with night blindness at 10 years old
  • Decreased central vision by 30 years old
  • Cataract requiring surgery in 50s
  • Current visual acuity 6/60 and 6/24
  • Bilateral, extensive bone spicule pigmentation
  • attenuated arterioles and pale optic discs with small preserved islands of RPE in macula

External Databases (Donor)

BioSamples SAMEA4458543

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 65-69
Passage number reprogrammed P4

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones Based on morphology, growth rate and expression of SSEA4 and NANOG
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SSEA-1
No
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
ACTA2
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No clinically significant imbalance was detected
Passage number: P30
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)