UNEWi026-B
Donor information | |
|---|---|
| Gender: | Male |
| Age: | 60-64 |
Disease status | |
| Disease: |
type 2 diabetes mellitus
Sporadic typical T2D |
| Disease variant | |
| Disease: |
Age-Related Macular Degeneration
Donor is at high risk of developing age-related macular degeneration but has not been diagnosed with AMD at time of biopsy. |
| Affected: | No |
| Carrier: | Yes |
| Disease variant | |
| Gene: | CFH |
| Chromosome location: | 1q31.3 |
| Nucleotide sequence HGVS: | NM_000186.3:c.1204C>T |
| Protein sequence HGVS: | NP_000177.2:p.His402Tyr NC_000001.11:g.19669010 |
| Zygosity: | Homozygous |
| ClinVar ID: | SCV000038294.5 |
| dbSNP ID: | rs1061170 |
General information | |
| Depositor: | University of Newcastle |
| Cell line name: | UNEWi026-B |
| Cell line alternative names: | SF116 clone 2 |
| Biosamples ID: | SAMEA4567590 |
| hPSCreg name: | UNEWi026-B |
| Derivation country: | United Kingdom |
Related lines | |
| From the same donor: | STBCi110-A, UNEWi026-A, UNEWi026-C |
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Derivation
Derivation
Primary cell type: Fibroblast of dermis Location of primary tissue procurement: Zone of skin Passage number reprogrammed: P4 Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: mTeSR Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 20% Temperature: 37C -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles TRA 1-60 Yes SSEA-4 Yes SSEA-1 No NANOG Yes Differentiation potency
Ectoderm: Ectoderm Marker Expressed TUJ-1 Yes Endoderm: Endoderm Marker Expressed Alpha FetoProtein Yes Mesoderm: Mesoderm Marker Expressed SMA Yes Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Karyotyping
Passage number: P11 Cell line karyotype: No clinically significant imbalance was detected Karyotyping method: Molecular karyotyping by SNP array Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
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