UNEWi018-A

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: 48 hours post-passage
Magnification: x4
Timepoint: 48 hours post-passage
Magnification: x10
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name UNEWi018-A
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University of Newcastle (UNEW)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg UNEWi018-A
BioSamples SAMEA3142165
Cellosaurus CVCL_II94
ECACC 66540012
CLO CLO_0101570
Wikidata Q54991159

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 10-14
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Aplastic anemia
The donor is affected.
Synonyms
  • Aplastic anaemia
Disease associated phenotypes
  • pancytopenia and hypocellular bone marrow
Family history No
Is the medical history available upon request? No
Is clinical information available? Idiopathic severe aplastic anaemia. Responded to rabbit Lymphoglobulin but then relapsed. Now in partial recovery following treatment with lymphocyte immune globulin

External Databases (Donor)

BioSamples SAMEA3142155

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 10-14

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
O2 Concentration 95 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-1
No
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
trisomy chromosome 3
Karyotyping method: CytoSNP

Other Genotyping (Cell Line)