UNEWi004-A

PRPF31 SH

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name UNEWi004-A
Alternative name(s)
PRPF31 SH
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University of Newcastle (UNEW)
Owner Institute of Genetic Medicine
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institute of Genetic Medicine

External Databases

hPSCreg UNEWi004-A
BioSamples SAMEA3275846
Cellosaurus CVCL_9S39
ECACC 66540017
CLO CLO_0101558
Wikidata Q54991154

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 45-49

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Retinitis pigmentosa
The donor is a carrier of a disease-associated mutation and affected.
Stage
Very Severe
Genetic variants
PRPF31 (target)
19q13.42
PRPF31: c.1115_1125 del11
Disease associated phenotypes
  • Retinitis Pigmentosa
  • Age of onset: 10 years old
  • presented with night blindness at 10 years old
  • decreased central vision by 30 years old
  • right optic nerve hypoplasia
  • current visual acuity - no perception of light right eye t and 6/18 left
  • bilateral extensive bone spicule pigmentation
  • attenuated arterioles and pale optic discs
  • visual field restricted to around 5 degrees from fixation
Family history two other members of the family show the same mutation with variable clinical phenotype

External Databases (Donor)

BioSamples SAMEA3275858

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 45-49

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Stem cell morphology and growth speed
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
Unknown

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)