UKKi036-B
Donor information | |
|---|---|
| Gender: | Female |
| Age: | 20-24 |
Disease status | |
| Disease: | normal |
General information | |
| Depositor: | Klinikum der Universität zu Köln |
| Cell line name: | UKKi036-B |
| Cell line alternative names: | NP0143-B, NP0143-15 |
| Biosamples ID: | SAMEA104615863 |
| hPSCreg name: | UKKi036-B |
| Derivation country: | Germany |
Related lines | |
| From the same donor: | UKKi036-A, UKKi036-C |
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Derivation
Derivation
Primary cell type: Peripheral blood mononuclear cell Tissue collection year: 2016 Passage number reprogrammed: 0 Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: No Merthods used: pcr Files showing reprogramming vector expressed or silenced: Image / Result Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Vitronectin Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 20% CO2 concentration: 5% Culture medium: other Culture base medium: StemFlex Main protein source: - Passage Number banked (pre-EBiSC): 15 No. Vials banked (pre-EBiSC): 25 -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles SSEA-4 Yes - - TRA 1-81 Yes - - NANOG Yes - OCT4 Yes - Morphology: 
Differentiation potency
Ectoderm: Ectoderm In vitro directed differentiation
Marker Expressed NES Yes Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdfEndoderm: Endoderm In vitro directed differentiation
Marker Expressed SOX17 Yes Morphology:
Mesoderm: Mesoderm In vitro directed differentiation
Marker Expressed BRACHYURY Yes Morphology:
Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative -
Genotyping
Genotyping
Karyotyping
Passage number: 12 Cell line karyotype: no larger chromosomal aberrations- Chr.20 early mosaicism of whole chromosome visible (20%) Karyotyping method: Molecular karyotyping by SNP array Image / Result
At European Collection of Authenticated Cell Cultures (ECACC)
Restocking
Cell Line Information Pack
A Cell Line Information Pack (CLIP) is created to communicate cell line specific information relating to restrictions on use to a user accessing cell lines from EBiSC. A CLIP contains information about a cell line including any specific third party obligations (TPOs) relating to, for example, intellectual property (IP) or the donor consent which affect the use of the cell line.