UKKi035-A

NP0139-A, NP0139-3E

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name UKKi035-A
Alternative name(s)
NP0139-A, NP0139-3E
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Klinikum der Universität zu Köln (UKK)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Germany

External Databases

hPSCreg UKKi035-A
BioSamples SAMEA104615859
ECACC 66540895
Cellosaurus CVCL_VF42
CLO CLO_0102290
Wikidata Q54990541

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 65-69

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Hypertrophic cardiomyopathy
MYBPC3; Exon 27; c.2827C>T; p.Arg943*
Genetic variants
MYBPC3 (target)
11p11.2
NM_000256.3:c.2827C>T
NP_000247.2:p.Arg943*
Heterozygous

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104615903

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 65-69
Collected in 2016
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex
Main protein source: Albumine
Serum concentration: 0 %
Supplements
StemFlex Supplement 10x 50 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
OCT 4
Yes
SSEA-4
Yes
TRA1-80
Yes
Morphology pictures
less colony attachment after thawing
growing still fine
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Yes
Morphology

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
no larger chromosomal aberrations
Passage number: 27

Other Genotyping (Cell Line)