UKKi031-C

NP0138-31B

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name UKKi031-C
Alternative name(s)
NP0138-31B
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Klinikum der Universität zu Köln (UKK)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Germany

External Databases

hPSCreg UKKi031-C
BioSamples SAMEA104494416
ECACC 66540880
Cellosaurus CVCL_RM76
CLO CLO_0101982
Wikidata Q54990529

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 55-59

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Hypertrophic cardiomyopathy (primary disease)
Tnnt2; Exon 9; c.281G>T; Arg94Leu
The donor is a carrier of a disease-associated mutation and affected.
Genetic variants
TNNT2 (target)
1q32.1
NM_001001430.2:c.281G>T
NP_001001430.1:p.Arg94Leu
Heterozygous
Please explain briefly the supporting evidence
Family history No
Is the medical history available upon request? yes
Is clinical information available? yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104494414

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 55-59
Collected in 2016
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex
Main protein source: Albumine
Serum concentration: 0 %
Supplements
StemFlex Supplement 10x 50 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-80
Yes
OCT 4
Yes
NANOG
Yes
SSEA-4
Yes
SOX2
Yes
FOXD3
Yes
DNMT3b
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Unknown
Morphology

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No larger chromosomal aberrations to be reported; Chr20: Duplication in q11.21
Passage number: 15
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes