UKKi031-B
Donor information | |
|---|---|
| Gender: | Female |
| Age: | 60-64 |
Disease status | |
| Disease: |
Hypertrophic cardiomyopathy
Tnnt2; Exon 9; c.281G>T; Arg94Leu |
| Affected: | Yes |
| Carrier: | Yes |
| Disease variant | |
| Gene: | TNNT2 |
| Chromosome location: | 1q32.1 |
| Nucleotide sequence HGSV: | NM_001001430.2:c.281G>T |
| Protein sequence HGSV: | NP_001001430.1:p.Arg94Leu |
| Zygosity: | Heterozygous |
| Description: | Please explain briefly the supporting evidence |
| Family history: | No |
| Medical history: | Yes |
| Clinical information: | Yes |
General information | |
| Depositor: | Klinikum der Universität zu Köln |
| Cell line name: | UKKi031-B |
| Cell line alternative names: | NP0138-19E |
| Biosamples ID: | SAMEA104494415 |
| hPSCreg name: | UKKi031-B |
| Derivation country: | Germany |
Related lines | |
| From the same donor: | UKKi031-A, UKKi031-C |
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Derivation
Derivation
Primary cell type: Peripheral blood mononuclear cell Tissue collection year: 2016 Passage number reprogrammed: 0 Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: No Merthods used: pcr Files showing reprogramming vector expressed or silenced: Image / Result Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Vitronectin Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 20% CO2 concentration: 5% Culture medium: other Culture base medium: StemFlex Main protein source: Albumine Passage Number banked (pre-EBiSC): 21 No. Vials banked (pre-EBiSC): 25 -
Characterization
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Genotyping
Genotyping
Karyotyping
Passage number: 18 Cell line karyotype: No larger chromosomal aberrations to be reported; Chr20: Duplication in q11.21. Karyotyping method: Molecular karyotyping by SNP array Image / Result
At European Collection of Authenticated Cell Cultures (ECACC)
Restocking
Cell Line Information Pack
A Cell Line Information Pack (CLIP) is created to communicate cell line specific information relating to restrictions on use to a user accessing cell lines from EBiSC. A CLIP contains information about a cell line including any specific third party obligations (TPOs) relating to, for example, intellectual property (IP) or the donor consent which affect the use of the cell line.