UKKi028-C
Donor information | |
|---|---|
| Gender: | Female |
| Age: | 30-34 |
| Ethnicity: | Chinese |
Disease status | |
| Disease: | normal |
General information | |
| Depositor: | Klinikum der Universität zu Köln |
| Cell line name: | UKKi028-C |
| Cell line alternative names: | NP0115-C |
| Biosamples ID: | SAMEA104381177 |
| hPSCreg name: | UKKi028-C |
| Derivation country: | Germany |
Related lines | |
| From the same donor: | UKKi028-A, UKKi028-B |
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Derivation
Derivation
Primary cell type: Peripheral blood mononuclear cell Tissue collection year: 2016 Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: No Merthods used: pcr Files showing reprogramming vector expressed or silenced: Image / Result Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Vitronectin Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 20% CO2 concentration: 5% Culture medium: other Culture base medium: StemFlex Main protein source: - Passage Number banked (pre-EBiSC): 24 No. Vials banked (pre-EBiSC): 25 -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles FOXD3 Yes - OCT 4 Yes - - NANOG Yes - - SSEA-1 Yes - SOX2 Yes - SSEA-4 Yes - - TRA 1-80 Yes - - DNMT3b Yes - CD30 Yes - Morphology: 
Differentiation potency
Ectoderm: Ectoderm In vitro directed differentiation
Marker Expressed NES Yes Morphology:
Endoderm: Endoderm In vitro directed differentiation
Marker Expressed SOX17 Yes Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdfMesoderm: Mesoderm In vitro directed differentiation
Marker Expressed BRACHYURY Yes Morphology:
Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative -
Genotyping
Genotyping
Karyotyping
Passage number: 27 Cell line karyotype: no larger chromosomal aberrations to be reported. Chr05: small deletion mosaicism on long arm Karyotyping method: Molecular karyotyping by SNP array Image / Result
At European Collection of Authenticated Cell Cultures (ECACC)
Restocking
Cell Line Information Pack
A Cell Line Information Pack (CLIP) is created to communicate cell line specific information relating to restrictions on use to a user accessing cell lines from EBiSC. A CLIP contains information about a cell line including any specific third party obligations (TPOs) relating to, for example, intellectual property (IP) or the donor consent which affect the use of the cell line.