UKKi028-A
Donor information | |
|---|---|
| Gender: | Female |
| Age: | 30-34 |
| Ethnicity: | Chinese |
Disease status | |
| Disease: | normal |
General information | |
| Depositor: | Klinikum der Universität zu Köln |
| Cell line name: | UKKi028-A |
| Cell line alternative names: | NP0115-A, NP0115-4 |
| Biosamples ID: | SAMEA104381174 |
| hPSCreg name: | UKKi028-A |
| Derivation country: | Germany |
Related lines | |
| From the same donor: | UKKi028-B, UKKi028-C |
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Derivation
Derivation
Primary cell type: Peripheral blood mononuclear cell Tissue collection year: 2016 Passage number reprogrammed: 0 Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: No Merthods used: pcr Files showing reprogramming vector expressed or silenced: Image / Result Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: - Passage method: - Matrix: - CO2 concentration: - O2 concentration: - Temperature: - -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles DNMT3b Yes - CD30 Yes - OCT 4 Yes - - FOXD3 Yes - SOX2 Yes - NANOG Yes - - SSEA-4 Yes - - SSEA-1 Yes - TRA 1-80 Yes - - Morphology: 
Differentiation potency
Ectoderm: Ectoderm In vitro directed differentiation
Marker Expressed NES Unknown Morphology:
Endoderm: Endoderm In vitro directed differentiation
Marker Expressed SOX17 Yes Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdfMesoderm: Mesoderm In vitro directed differentiation
Marker Expressed BRACHYURY Yes Morphology:
Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Karyotyping
Passage number: 10 Cell line karyotype: no larger chromosomal aberrations to be reported Image / Result Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
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