UKKi025-C

NP0135-7

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name UKKi025-C
Alternative name(s)
NP0135-7
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Germany

External Databases

hPSCreg UKKi025-C
BioSamples SAMEA17638918
Cellosaurus CVCL_LD44
ECACC 66540493
CLO CLO_0101635
Wikidata Q54990493

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 40-44
Ethnicity caucasian / european

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Hypertrophic cardiomyopathy
MYH7, Exon 19, heterozygous mutation, c.2156G>A, p.Arg719Gln
The donor is a carrier of a disease-associated mutation and affected.
Genetic variants
MYH7 (target)
14q11.2
NM_000257.4:c.2156G>A
NP_000248.2:p.Arg719Gln
Heterozygous
Please explain briefly the supporting evidence
Disease associated phenotypes
  • atrioventricular node ablation
  • atrial tachycardia
  • auricular fibrillation
  • cardinal decompensation
Family history the mother is also affected
Is clinical information available? Mutation: MYH7(Exon19); c.2156G>A; p.Arg719Gly

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
No larger chromosomal aberrations observed
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA17637418

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 40-44
Collected in 2016
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection sendai virus absent
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Supplements
Essential 8 supplement 50x 10 ml
Essential 8 Flex supplement 50x 10 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-1
Yes
SSEA-4
Yes
TRA 1-80
Yes
SOX2
Yes
OCT 4
Yes
DNMT3b
Yes
FOXD3
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
expression of SOX17 in differentiated iPS cell line NP0135-7
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
expression of BRACHYURY in differentiated iPS cell line NP0135-7
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Yes
Morphology
expression of NESTIN in differentiated iPS cell line NP0135-7

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 37

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No larger chromosomal aberrations observed.
Passage number: 31
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array