UKKi024-B
NP0133-1
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.
General#
Cell Line |
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| hPSCreg Name | UKKi024-B |
| Alternative name(s) |
NP0133-1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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| Depositor | Klinikum der Universität zu Köln (UKK) |
| Owner | Institute for Neurophysiology, Medical Faculty |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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| Derivation country | Germany |
External Databases |
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| hPSCreg | UKKi024-B |
| BioSamples | SAMEA17635168 |
| Cellosaurus | CVCL_LD85 |
| ECACC | 66540489 |
| CLO | CLO_0101631 |
| Wikidata | Q54990488 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: allowed
Commercial: allowed
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Donor Information#
General Donor Information |
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| Sex | male |
| Age of donor (at collection) | 30-34 |
| Ethnicity | caucasian / european |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Family history | death from heart disease of the uncle |
| Is clinical information available? | electrocardiogram typical for patients with Brugada syndrome type 1, asympthomatic demasking in ajmalin test |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA17634418 |
hIPSC Derivation#
General |
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| Source cell type |
peripheral blood mononuclear cellA leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
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| Age of donor (at collection) | 30-34 |
| Collected in | 2016 |
| Passage number reprogrammed | 0 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
| Notes on reprogramming vector detection | sendai virus absent |
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Selection criteria for clones | morphology |
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Vitronectin | |||||||||
| Feeder cells |
No |
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| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 20 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Essential 8™
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
|||||||||
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Morphology pictures
thawing control of NP0133-1
thawing control of NP0133-1
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| SOX17 |
Yes |
Morphology
expression of SOX17 in differentiated iPS cell line NP0133-1
In vitro directed differentiation
| Marker | Expressed |
| BRACHYURY |
Yes |
Morphology
expression of BRACHYURY in differentiated iPS cell line NP0133-1
In vitro directed differentiation
| Marker | Expressed |
| NES |
Yes |
Morphology
expression of NESTIN in differentiated iPS cell line NP0133-1
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Sterility |
|
| Inoculation for microbiological growth | No Contaminants Detected |
| Mycoplasma | Not Detected |
| Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
No larger chromosomal aberrations observed
Passage number: 32
Karyotyping method:
Molecular karyotyping by SNP array
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Other Genotyping (Cell Line) |
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