UKKi022-C

• Derivation

  Derivation
  Primary cell type:	Fibroblast of dermis
  Location of primary tissue procurement:	Zone of skin
  Tissue collection year:	2016
  Passage number reprogrammed:	3
  Reprogramming method
  Vector type:	Non-integrating
  Vector:	Episomal
  Is the reprogramming vector detectable:	No
  Merthods used:	pcr
  Notes on reprogramming vector detection:	episomal vector cassette absent
  Files showing reprogramming vector expressed or silenced:	Image / Result
  Derivation
  Xeno free conditions:	no
  Derived under gmp:	no
  Available as clinical grade:	no

• Culture conditions

  Culture Conditions
  Medium:	-
  Passage method:	-
  Matrix:	-
  CO2 concentration:	-
  O2 concentration:	-
  Temperature:	-

• Characterization

  Characterization
  Analysis of Undifferentiated Cells
  Marker expression:	Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles DNMT3b Yes - CD90 Yes - SSEA-4 Yes - - TRA 1-80 Yes - - FOXD3 Yes - SOX2 Yes - SSEA-1 Yes - NANOG Yes - - OCT 4 Yes - -
  Morphology:	morphology pictures of iPS cell line NP0106-10A before banking and of the thawing control morphology pictures of iPS cell line NP0106-10A before banking and of the thawing control morphology pictures of iPS cell line NP0106-10A before banking and of the thawing control
  Differentiation potency
  Ectoderm:	Ectoderm In vitro directed differentiation Marker Expressed NES Yes Morphology: expression of NESTIN in differentiated iPS cell line NP0106-10
  Endoderm:	Endoderm In vitro directed differentiation Marker Expressed SOX17 Unknown Morphology: expression of SOX17 in differentiated iPS cell line NP0106-10
  Mesoderm:	Mesoderm In vitro directed differentiation Marker Expressed BRACHYURY Yes Morphology: expression of BRACHYURY in differentiated iPS cell line NP0106-10
  Microbiology / Virus Screening
  HIV 1:	Negative
  HIV 2:	Negative
  Hepatitis B:	Negative
  Hepatitis C:	Negative
  Mycoplasma:	Negative
  Sterility
  Inoculation for microbiological growth:	No Contaminants Detected
  Mycoplasma:	Not Detected
  Viability:	Viable post-cryopreservation

• Genotyping

  Genotyping
  Karyotyping
  Passage number:	17
  Cell line karyotype:	Chr10: 3Mbp large duplication mosaicism on short arm.
  Karyotyping method:	Molecular karyotyping by SNP array
  	Image / Result
  Genotyping
  Genome-wide analysis:	Whole genome sequencing https://ega-archive.org/studies/EGAS00001002755
  STR/Fingerprinting:	A 16 allele profile has been recorded and data is available upon request, after cell line purchase.