UKKi018-B
Donor information | |
|---|---|
| Gender: | Female |
| Age: | 20-24 |
| Ethnicity: | Caucasian / european |
Disease status | |
| Disease: |
Familial long QT syndrome
Disease variant: familial long QT syndrome 2, KCNH2, c.173 A>G; p.Glu58Gly |
| Affected: | Yes |
| Carrier: | Yes |
| Disease variant | |
| Gene: | KCNH2 |
| Chromosome location: | 7q36.1 |
| Nucleotide sequence HGVS: | NM_000238.3:c.173 A>G |
| Protein sequence HGVS: | NP_000229.1:p.Glu58Gly |
| Zygosity: | Heterozygous |
| Description: | Please explain briefly the supporting evidence |
| Medical history: | Yes |
| Clinical information: | Yes |
General information | |
| Depositor: | Klinikum der Universität zu Köln |
| Cell line name: | UKKi018-B |
| Cell line alternative names: | NP0080-6A |
| Biosamples ID: | SAMEA17623918 |
| hPSCreg name: | UKKi018-B |
| Derivation country: | Germany |
Related lines | |
| From the same donor: | UKKi018-A, UKKi018-C |
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Derivation
Derivation
Primary cell type: Peripheral blood mononuclear cell Primary cell line vendor: University Hospital Münster, Germany Tissue collection year: 2016 Passage number reprogrammed: 0 Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: No Merthods used: immune_staining, pcr Notes on reprogramming vector detection: sendai virus absent Files showing reprogramming vector expressed or silenced: Image / Result Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: - Passage method: - Matrix: - CO2 concentration: - O2 concentration: - Temperature: - -
Characterization
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Genotyping
Genotyping
Karyotyping
Passage number: 34 Cell line karyotype: No larger chromosomal aberrations observed Karyotyping method: Molecular karyotyping by SNP array Image / Result Genotyping
Genome-wide analysis: SNP typing array
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
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