|Disease:||Spinocerebellar ataxia type 3|
|Disease stage:||Scores on the Scale for the Assessment and Rating of Ataxia (SARA, range: 0 – 40) was 22.5|
|Disease associated phenotypes:||Symptomatic, 7 years post disease onset., ataxia, wheelchair-bound; in addition to ataxia: parkinsonism, restless legs syndrome (RLS), spasticity, facial dystonia, and neuropathy.|
|Family history:||De novo mutation|
|Cell line name:||UKBi003-A|
|Cell line alternative names:||iLB-MJD1-32m-r9, LB-32-r9|
Primary cell type: Fibroblast of dermis Location of primary tissue procurement: Zone of skin Tissue collection year: 2008 Passage number reprogrammed: P4
Vector type: Integrating Vector: Virus Virus type: Retrovirus Gene list:
Absence of reprogramming vector(s): yes Have the reprogramming vectors been silenced: Yes Merthods used: pcr Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no
Medium: mTeSR Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 21% Temperature: 37C
Passage number: 28 Cell line karyotype: XY Karyotyping method: Molecular karyotyping by SNP array Image / Result
SNP typing array
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Cell Line Information Pack
A Cell Line Information Pack (CLIP) is created to communicate cell line specific information relating to restrictions on use to a user accessing cell lines from EBiSC. A CLIP contains information about a cell line including any specific third party obligations (TPOs) relating to, for example, intellectual property (IP) or the donor consent which affect the use of the cell line.