UKBi001-B

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name UKBi001-B
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Universitätsklinikum Bonn (UKB)
Owner Universitätsklinikum Bonn
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institut für Rekonstruktive Neurobiologie
Scottish Centre for Regenerative Medicine
Derivation country Germany

External Databases

hPSCreg UKBi001-B
BioSamples SAMEA3855072
Cellosaurus CVCL_W573
ECACC 66540043
CLO CLO_0101562
Wikidata Q54905792

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 40-44
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Spinocerebellar ataxia type 3
The donor is affected.
Stage
Scores on the Scale for the Assessment and Rating of Ataxia (SARA, range: 0 – 40) was 23.
Synonyms
  • Nigro-spino-dentatal degeneration with nuclear ophthalmoplegia
  • Machado-Joseph disease
  • SCA3
  • MJD
  • Machado disease
  • Azorean disease of the nervous system
show more synonyms
Genetic variants
ATXN3 (target)
14q32.12
Heterozygous
PMID: 22113611
ATXN3: CAG repeat length of 74/21
Family history de novo mutation

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives
Has father:

External Databases (Donor)

BioSamples SAMEA2614020

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 40-44
Collected in 2008
Passage number reprogrammed p4

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro directed differentiation
Neuroepithelial Stem Cell
Ont Id: CL_0002259
In vivo teratoma
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46xy
Passage number: 13
Karyotyping method: SNP-karyotyping

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes