UHOMi001-A

iPCD02.30

iPSC line

The cell line is not available.

General#

Cell Line

hPSCreg Name UHOMi001-A
Alternative name(s)
iPCD02.30
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University Hospital of Montpellier (UHOM)
Owner University Hospital of Montpellier (UHOM)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country France

External Databases

hPSCreg UHOMi001-A
Cellosaurus CVCL_VF21
BioSamples SAMEA8477514
CLO CLO_0103218
Wikidata Q54990170

General Information

* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Lung research and development
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Primary ciliary dyskinesia
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • PCD
Genetic variants
ccdc40 (target)
Is the medical history available upon request? Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA8477515

hIPSC Derivation#

General

Source cell type
dermal fibroblast
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-81
Yes
NANOG
Yes
TRA 1-60
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Marker Expressed
CDX2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Marker Expressed
VIM
Yes
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
Morphology
Pigmented neural tubes (Teratoma)

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)