UCLi006-A

LCMD-L302P-UCL01C2

iPSC line

The cell line is not available.

General#

Cell Line

hPSCreg Name UCLi006-A
Alternative name(s)
LCMD-L302P-UCL01C2
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University College London (UCL)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg UCLi006-A
BioSamples SAMEA104130970
Cellosaurus CVCL_VF11
CLO CLO_0101660
Wikidata Q54989674

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Congenital muscular dystrophy due to LMNA mutation (primary disease)
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • LMNA-related congenital muscular dystrophy
  • L-CMD
Genetic variants
LMNA (target)
1q21.1-21.2
c.905T>C
p.Leu302Pro
Heterozygous
18551513

External Databases (Donor)

BioSamples SAMEA104130971

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Yes
Methods used
Immunostaining, RT-PCR
Vector map

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Alkaline Phosphatase
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
Alpha FetoProtein
Yes
FOXA2
Yes
GATA Binding Protein 4
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
MSX1
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
MAP2
Yes
PAX6
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal
Karyotyping method: G-Banding

Other Genotyping (Cell Line)