STBCi110-A
Donor information | |
|---|---|
| Gender: | Male |
Disease status | |
| Disease: |
type 2 diabetes mellitus
Sporadic typical T2D |
| Disease variant | |
| Disease: |
Age-Related Macular Degeneration
Donor is at high risk of developing age-related macular degeneration but has not been diagnosed with AMD at time of biopsy. |
| Affected: | No |
| Carrier: | Yes |
| Disease variant | |
| Gene: | CFH |
| Chromosome location: | 1q31.3 |
| Nucleotide sequence HGVS: | NM_000186.3:c.1204C>T |
| Protein sequence HGVS: | NP_000177.2:p.His402Tyr NC_000001.11:g.19669010 |
| Zygosity: | Homozygous |
| ClinVar ID: | SCV000038294.5 |
| dbSNP ID: | rs1061170 |
General information | |
| Depositor: | StemBANCC |
| Cell line name: | STBCi110-A |
| Cell line alternative names: | SFC116-03-01 |
| Biosamples ID: | SAMEA104616143 |
| hPSCreg name: | STBCi110-A |
Related lines | |
| From the same donor: | UNEWi026-A, UNEWi026-B, UNEWi026-C |
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Derivation
Derivation
Primary cell type: Fibroblast Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Is the reprogramming vector detectable: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Matrigel/Geltrex Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 21% CO2 concentration: 5% Culture medium: mTeSR™ 1 -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles NANOG Yes TRA 1-60 Yes Microbiology / Virus Screening
HIV 1: - HIV 2: - Hepatitis B: - Hepatitis C: - Mycoplasma: Negative -
Genotyping
Genotyping
Karyotyping
Passage number: 8 Cell line karyotype: No abnormalities detected Karyotyping method: Molecular karyotyping by SNP array
At European Collection of Authenticated Cell Cultures (ECACC)
Restocking
Cell Line Information Pack
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A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.