STBCi083-B
Donor information | |
|---|---|
| Gender: | Male |
Disease status | |
| Disease: | Parkinson's disease |
| Disease variant | |
| Description: | SNCA A53T |
General information | |
| Depositor: | StemBANCC |
| Cell line name: | STBCi083-B |
| Cell line alternative names: | SFC830-04-08 |
| Biosamples ID: | SAMEA104493877 |
| hPSCreg name: | STBCi083-B |
Related lines | |
| From the same donor: | STBCi083-A |
Timepoint: confluence
Magnification: 4x
Timepoint: confluence
Magnification: 10x
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Derivation
Derivation
Primary cell type: Fibroblast Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Is the reprogramming vector detectable: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: mTeSR-1 Passage method: EDTA Matrix: Matrigel CO2 concentration: 5% O2 concentration: 20% Temperature: 37C -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles POU5F1 (OCT-4) Yes TRA 1-60 Yes SSEA-4 Yes SSEA-1 No Differentiation potency
Ectoderm: Ectoderm In vitro spontaneous differentiation
Endoderm: Endoderm In vitro spontaneous differentiation
Mesoderm: Mesoderm In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: - HIV 2: - Hepatitis B: - Hepatitis C: - Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Karyotyping
Cell line karyotype: No abnormalities detected Karyotyping method: Molecular karyotyping by SNP array Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
Cell Line Information Pack
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A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
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