STBCi037-A

SFC816-03-02

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name STBCi037-A
Alternative name(s)
SFC816-03-02
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg STBCi037-A
BioSamples SAMEA104493691
Cellosaurus CVCL_RC12
CLO CLO_0101769
Wikidata Q54956452

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 60-64

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
neuropathy (primary disease)
Genetic variants
GARS S581L

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493692

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 60-64

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
To be confirmed
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)