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Due to the ongoing COVID19 crisis, EBiSC is restricting non-essential work across all central biobanking sites to try and help slow spread of the disease. During this time, to minimise staff numbers within our facilities, cell lines will not be shipped. If you have ordered lines, these orders will be shipped as soon as possible after local restrictions are lifted. EBiSC can continue to help you select appropriate iPSC lines for your research and you can still apply to access managed access datasets. If you have any queries about new or ongoing orders, please contact ECACC at CultureCollections@phe.gov.uk or contact EBiSC directly.

STBCi026-C

Donor information
Gender:	Female
Disease status
Disease:	normal
General information
Depositor:	StemBANCC
Cell line name:	STBCi026-C
Cell line alternative names:	SFC840-03-05
Biosamples ID:	SAMEA104493718
hPSCreg name:	STBCi026-C
Related lines
From the same donor:	STBCi026-A, STBCi026-B, STBCi026-D

Derivation

Derivation
Primary cell type:	Fibroblast
Reprogramming method
Vector type:	Non-integrating
Vector:	Sendai virus
Is the reprogramming vector detectable:	Unknown
Derivation
Xeno free conditions:	no
Derived under gmp:	no
Available as clinical grade:	no

Culture conditions

Culture Conditions
Surface coating:	Matrigel/Geltrex
Passage method:	Enzyme-free cell dissociation / EDTA
O₂ concentration:	21%
CO₂ concentration:	5%
Culture medium:	mTeSR™ 1

Characterization

Characterization

Analysis of Undifferentiated Cells

Marker expression:

Marker	Expressed	Immunostaining	RT-PCR	FACS	Enzymatic Assay	Expression Profiles
NANOG	Yes
TRA 1-60	Yes

Microbiology / Virus Screening

HIV 1:

-

HIV 2:

-

Hepatitis B:

-

Hepatitis C:

-

Mycoplasma:

Negative

Genotyping

Genotyping
Karyotyping
Cell line karyotype:	none detected by StemDB pipeline, though Karyostudio analysis shows 6 small changes (SC 08.06.2015 edit)
Karyotyping method:	Molecular karyotyping by SNP array

At European Collection of Authenticated Cell Cultures (ECACC)