STBCi026-C
Donor information | |
|---|---|
| Gender: | Female |
Disease status | |
| Disease: | normal |
General information | |
| Depositor: | StemBANCC |
| Cell line name: | STBCi026-C |
| Cell line alternative names: | SFC840-03-05 |
| Biosamples ID: | SAMEA104493718 |
| hPSCreg name: | STBCi026-C |
Related lines | |
| From the same donor: | STBCi026-A, STBCi026-B, STBCi026-D |
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Derivation
Derivation
Primary cell type: Fibroblast Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Is the reprogramming vector detectable: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Matrigel/Geltrex Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 21% CO2 concentration: 5% Culture medium: mTeSR™ 1 -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles TRA 1-60 Yes NANOG Yes Microbiology / Virus Screening
HIV 1: - HIV 2: - Hepatitis B: - Hepatitis C: - Mycoplasma: Negative -
Genotyping
Genotyping
Karyotyping
Cell line karyotype: none detected by StemDB pipeline, though Karyostudio analysis shows 6 small changes (SC 08.06.2015 edit) Karyotyping method: Molecular karyotyping by SNP array
At European Collection of Authenticated Cell Cultures (ECACC)
Restocking
Cell Line Information Pack
A Cell Line Information Pack (CLIP) is created to communicate cell line specific information relating to restrictions on use to a user accessing cell lines from EBiSC. A CLIP contains information about a cell line including any specific third party obligations (TPOs) relating to, for example, intellectual property (IP) or the donor consent which affect the use of the cell line.