STBCi026-A-4

SFC840-03-03-PITX3-GFP

Gene-edited iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name STBCi026-A-4
Alternative name(s)
SFC840-03-03-PITX3-GFP
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg STBCi026-A-4
BioSamples SAMEA5859482
CLO CLO_0103204
Cellosaurus CVCL_A4YP
Wikidata Q107116956

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493661

hIPSC Derivation#

General

The source cell information can be found in the parental cell line STBCi026-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1

Characterisation#

Differentiation Potency
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Marker Expressed
TUJ-1
Yes
PITX3
Yes
Morphology
Dopaminergic neuronal differentiation

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
PITX3 (target)
Gene knock-in
10q24.32
GFP
10q24.32
PITX3-GFP tag inserted, single copy.
CRISPR-associated (CRISPR/Cas) System