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STBCi017-A

SFC811-03-01

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
To receive the Certificate of Analysis, please contact us.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name STBCi017-A
Alternative name(s)
SFC811-03-01
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi017-B
(SFC811-03-04)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-C
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SCN9A
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neuropathy
STBCi036-A
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SCN9A
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neuropathy
STBCi035-A
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SCN9A
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neuropathy
STBCi035-B
(SFC813-03-03)
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SCN9A
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neuropathy
STBCi035-C
(SFC813-03-07)
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SCN9A
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neuropathy
STBCi129-A
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SCN9A
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RCi001-A
(Thp3C-6, RCi88)
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SCN9A, SCN9A
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RCi001-B
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SCN9A, SCN9A
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RCi002-A
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SCN9A, SCN9A
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STBCi060-A
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neuropathy
STBCi060-B
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neuropathy
STBCi182-A
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neuropathy
STBCi184-A
(SFC251-03-01)
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neuropathy
STBCi018-B
(SFC815-03-08)
Donor's gene variants:
GARS1
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neuropathy
STBCi018-C
(SFC815-03-02)
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GARS1
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neuropathy
STBCi225-A
(SFC365-03-01)
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neuropathy
STBCi232-A
(SFC375-03-01)
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neuropathy
STBCi049-A
(SFC040-03-02)
Donor diseases:
neuropathy
STBCi049-B
(SFC040-03-03)
Donor diseases:
neuropathy
STBCi050-A
(SFC041-04-01)
Donor diseases:
neuropathy
STBCi050-B
(SFC041-04-02)
Donor diseases:
neuropathy
STBCi049-C
(SFC040-03-01)
Donor diseases:
neuropathy
STBCi050-C
(SFC041-04-03)
Donor diseases:
neuropathy
STBCi181-A
(SFC247-03-01)
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STBCi183-A
(SFC250-03-01)
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STBCi195-A
(SFC286-03-01)
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Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC

External Databases

hPSCreg STBCi017-A
BioSamples SAMEA104493629
Cellosaurus CVCL_RB65
Wikidata Q54956380

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclones

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 25-29

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Genetic variants
SCN9A (target)
SCN9A heterozygotes for a) Premature stop codon in coding exon 15 p.R830X and b) A 1 bp deletion within coding exon 26 which induces a frameshift at position 1773 in the C terminal domain of the channel c.2488C>T

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493630

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 25-29

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)