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STBCi006-A-3

ApoE 3/3

Gene-edited iPSC line

The cell line was withdrawn.

General#

Cell Line
hPSCreg Name STBCi006-A-3
Alternative name(s)
ApoE 3/3
Cell line type Human induced pluripotent stem cell (hiPSC)
Notes This line is part of a set of isogenic APOE lines based on the iPS cell line STBCi006-A. The set comprises the following APOE genotypes: • STBCi006-A-3 (APOE 3/3) • STBCi006-A-4 (APOE 3/4) • STBCi006-A (APOE 4/4) • STBCi006-A-1 (APOE KO) A DNA SNP array revealed a duplication of 2 MB on chromosome 20q, with significant genes involved (refer to “SNP typing array” in the Genotyping section for more information). This duplication was found in all isogenic lines generated from STBCi006-A. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, BIONi37-A and UKBi011-A.

Provider
Depositor Bioneer (BION)
Owner Bioneer (BION)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases
hPSCreg STBCi006-A-3
BioSamples SAMEA104243172
Cellosaurus CVCL_RX82
CLO CLO_0102754
Wikidata Q54956334

General Information
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed
Subclone of

Donor Information#

General Donor Information
Sex female

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Alzheimer disease
Alzheimer disease in the OLS
The donor is a carrier of a disease-associated mutation and affected.
Genetic variants
APOE (target)
19q13.3
NM_000041.4:c.388T>C
NP_000032.1:p.Cys130Arg
Homozygous
SCV000039748.4
rs429358
The genotype at base position described by rs429358 is C/C, coding Arg, contributing to ApoE4/E4 variant
APOE (target)
19q13.32
NM_000041.3:c.526C
NP_000032.1:p.Arg176
Homozygous
rs7412
The genotype at base position described by rs7412 is C/C, coding Arg, contributing to ApoE4/E4 variant.

External Databases (Donor)
BioSamples SAMEA104133819

hIPSC Derivation#

General
The source cell information can be found in the parental cell line STBCi006-A.

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46, XX
GW20170291.011_.K_Karyotype_STBCi006-A-3_.JPG
Passage number: 28
Karyotyping method: G-Banding

Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?
Yes
SNP typing array
SNP array done by Life&Brain
vcf File:
SNP Analysis_STBCi006-A-3.pdf

Genetic Modification#

Disease/phenotype related modifications
Alzheimer disease
Alzheimer disease in the OLS
Genetic modifications
APOE (target)
Isogenic modification
19q13.32
NM_000041.4:c.388C>T
NP_000032.1:p.Arg130Cys
Homozygous
STBCi006-A-3 ApoE3/E3 is an isogenic mutant of STBCi006-A (ApoE4/E4); The genotype at base position described by rs429358 has been modified in the subclone from C/C to T/T, which changes aa from Arg to Cys. Together with a C/C genotype at rs7412 these define a ApoE3/E3 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.
Changed