STBCi006-A-3
ApoE 3/3
Gene-edited iPSC line
The cell line was withdrawn.
General#
Cell Line |
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| hPSCreg Name | STBCi006-A-3 |
| Alternative name(s) |
ApoE 3/3
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Notes | This line is part of a set of isogenic APOE lines based on the iPS cell line STBCi006-A. The set comprises the following APOE genotypes: • STBCi006-A-3 (APOE 3/3) • STBCi006-A-4 (APOE 3/4) • STBCi006-A (APOE 4/4) • STBCi006-A-1 (APOE KO) A DNA SNP array revealed a duplication of 2 MB on chromosome 20q, with significant genes involved (refer to “SNP typing array” in the Genotyping section for more information). This duplication was found in all isogenic lines generated from STBCi006-A. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, BIONi37-A and UKBi011-A. |
Provider |
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| Depositor | Bioneer (BION) |
| Owner | Bioneer (BION) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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External Databases |
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| hPSCreg | STBCi006-A-3 |
| BioSamples | SAMEA104243172 |
| ECACC | 66540615 |
| Cellosaurus | CVCL_RX82 |
| CLO | CLO_0102754 |
| Wikidata | Q54956334 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: allowed
Commercial: allowed
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| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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External Databases (Donor) |
|
| BioSamples | SAMEA104133819 |
hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
STBCi006-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 18 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46, XX
Passage number: 28
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
SNP array done by Life&Brain vcf File: |
Genetic Modification#
| Disease/phenotype related modifications |
|