STBCi006-A-3 Gene-edited iPSC line
Donor information | |
|---|---|
| Gender: | Female |
Disease status | |
Donor | |
| Disease: | Alzheimer's disease |
| Affected: | Yes |
| Carrier: | Yes |
| Disease variant | |
| Gene: | ApoE |
| Chromosome location: | 19q13.3 |
| Nucleotide sequence HGVS: | NM_000041.4:c.388T>C |
| Protein sequence HGVS: | NP_000032.1:p.Cys130Arg |
| Zygosity: | Homozygous |
| ClinVar ID: | SCV000039748.4 |
| dbSNP ID: | rs429358 |
| Description: | The genotype at base position described by rs429358 is C/C, coding Arg, contributing to ApoE4/E4 variant |
| Disease variant | |
| Gene: | ApoE |
| Chromosome location: | 19q13.32 |
| Nucleotide sequence HGVS: | NM_000041.3:c.526C |
| Protein sequence HGVS: | NP_000032.1:p.Arg176 |
| Zygosity: | Homozygous |
| dbSNP ID: | rs7412 |
| Description: | The genotype at base position described by rs7412 is C/C, coding Arg, contributing to ApoE4/E4 variant. |
Disease/phenotype related genetic modifications |
|
| Disease: | Alzheimer's disease |
| Type of modification: | Isogenic |
| Gene: | ApoE |
| Chromosome location: | 19q13.32 |
| Nucleotide sequence HGVS: | NM_000041.4:c.388C>T |
| Protein sequence HGVS: | NP_000032.1:p.Arg130Cys |
| Zygosity: | Homozygous |
| Target locus modification: | Changed |
| Description: | STBCi006-A-3 ApoE3/E3 is an isogenic mutant of STBCi006-A (ApoE4/E4); The genotype at base position described by rs429358 has been modified in the subclone from C/C to T/T, which changes aa from Arg to Cys. Together with a C/C genotype at rs7412 these define a ApoE3/E3 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone. |
General information | |
| Depositor: | Bioneer |
| Cell line name: | STBCi006-A-3 |
| Cell line alternative names: | ApoE 3/3 |
| Biosamples ID: | SAMEA104243172 |
| hPSCreg name: | STBCi006-A-3 |
Related lines | |
| Derived from: | STBCi006-A |
| All subclones of STBCi006-A: | STBCi006-A-1, STBCi006-A-3, STBCi006-A-4 |
This line is part of a set of isogenic APOE lines based on the iPS cell line STBCi006-A. The set comprises the following APOE genotypes:
• STBCi006-A-3 (APOE 3/3)
• STBCi006-A-4 (APOE 3/4)
• STBCi006-A (APOE 4/4)
• STBCi006-A-1 (APOE KO)
A DNA SNP array revealed a duplication of 2 MB on chromosome 20q, with significant genes involved (refer to “SNP typing array” in the Genotyping section for more information). This duplication was found in all isogenic lines generated from STBCi006-A. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, BIONi37-A and UKBi011-A.
-
Derivation
Derivation
Primary cell type: - Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Is the reprogramming vector detectable: No Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Matrigel/Geltrex Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 18% CO2 concentration: 5% Culture medium: Essential 8™ Passage Number banked (pre-EBiSC): 37 No. Vials banked (pre-EBiSC): 30 -
Characterization
Characterization
Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative -
Genotyping
Genotyping
Karyotyping
Passage number: 28 Cell line karyotype: 46, XX Karyotyping method: G-Banding Image / Result Genotyping
Genome-wide analysis: SNP typing array
Data file -
Genetic modification
Genetic modification
Disease/phenotype related modifications
Disease: Alzheimer's disease Type of modification: Isogenic Gene: ApoE Chromosome location: 19q13.32 Nucleotide sequence HGVS: NM_000041.4:c.388C>T Protein sequence HGVS: NP_000032.1:p.Arg130Cys Zygosity: Homozygous Target locus modification: Changed Description: STBCi006-A-3 ApoE3/E3 is an isogenic mutant of STBCi006-A (ApoE4/E4); The genotype at base position described by rs429358 has been modified in the subclone from C/C to T/T, which changes aa from Arg to Cys. Together with a C/C genotype at rs7412 these define a ApoE3/E3 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.
At European Collection of Authenticated Cell Cultures (ECACC)
Restocking
Cell Line Information Pack
A Cell Line Information Pack (CLIP) is created to communicate cell line specific information relating to restrictions on use to a user accessing cell lines from EBiSC. A CLIP contains information about a cell line including any specific third party obligations (TPOs) relating to, for example, intellectual property (IP) or the donor consent which affect the use of the cell line.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.