SIGi001-A-13 Gene-edited iPSC line
Donor information | |
|---|---|
| Gender: | Female |
| Ethnicity: | Caucasian |
Disease status | |
Donor | |
| Disease: | normal |
| Family history: | No |
| Medical history: | No |
| Clinical information: | No |
Disease/phenotype related genetic modifications |
|
| Disease: | Alzheimer's disease |
| Type of modification: | Isogenic |
| Gene: | MAPT |
| Chromosome location: | 17q21.31 |
| Zygosity: | Heterozygous |
| Target locus modification: | Mutated |
| Description: | MAPT 10+16T Mono allelic |
| Disease: | Frontotemporal dementia |
General information | |
| Depositor: | Sigma-Aldrich |
| Cell line name: | SIGi001-A-13 |
| Cell line alternative names: | iPSC0028 – MonoAllelic MAPT_Ex10+16T/Clone 1D01-11 |
| Biosamples ID: | SAMEA104386250 |
| hPSCreg name: | SIGi001-A-13 |
Related lines | |
| Derived from: | SIGi001-A |
| All subclones of SIGi001-A: | SIGi001-A-1, SIGi001-A-10, SIGi001-A-11, SIGi001-A-12, SIGi001-A-13, SIGi001-A-2, SIGi001-A-3, SIGi001-A-4, SIGi001-A-5, SIGi001-A-6, SIGi001-A-7, SIGi001-A-8, SIGi001-A-9 |
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
-
Derivation
Derivation
Primary cell type: - Reprogramming method
Vector type: Integrating Vector: Virus Virus type: Retrovirus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Have the reprogramming vectors been silenced: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: mTeSR-1 Passage method: EDTA Matrix: Matrigel CO2 concentration: 5% O2 concentration: 20% Temperature: 37C -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles SSEA-3 Yes TRA 1-60 Yes POU5F1 (OCT-4) Yes Differentiation potency
Ectoderm: Ectoderm In vitro spontaneous differentiation
Endoderm: Endoderm In vitro spontaneous differentiation
Mesoderm: Mesoderm In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: - HIV 2: - Hepatitis B: - Hepatitis C: - Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Karyotyping
Cell line karyotype: 46, XX Karyotyping method: G-Banding Genotyping
Genome-wide analysis: Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase. -
Genetic modification
Genetic modification
Disease/phenotype related modifications
Disease: Alzheimer's disease Type of modification: Isogenic Gene: MAPT Chromosome location: 17q21.31 Zygosity: Heterozygous Target locus modification: Mutated Description: MAPT 10+16T Mono allelic Disease: Frontotemporal dementia
The parental iPSC line SIGi001-A can be sourced via Sigma as 'iPSC0028'.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
Cell Line Information Pack
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