SIGi001-A-1 Gene-edited iPSC line
Donor information | |
|---|---|
| Gender: | Female |
| Ethnicity: | Caucasian |
Disease status | |
| Disease: | normal |
| Family history: | No |
| Medical history: | No |
| Clinical information: | No |
General information | |
| Depositor: | Sigma-Aldrich |
| Cell line name: | SIGi001-A-1 |
| Cell line alternative names: | iPSC0028 SLC17A7/GFP E3 |
| Biosamples ID: | SAMEA4451096 |
| hPSCreg name: | SIGi001-A-1 |
Related lines | |
| Derived from: | SIGi001-A |
| All subclones of SIGi001-A: | SIGi001-A-1, SIGi001-A-10, SIGi001-A-11, SIGi001-A-12, SIGi001-A-13, SIGi001-A-2, SIGi001-A-3, SIGi001-A-4, SIGi001-A-5, SIGi001-A-6, SIGi001-A-7, SIGi001-A-8, SIGi001-A-9 |
The parental iPSC line SIGi001-A can be sourced via Sigma as 'iPSC0028'.
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Derivation
Derivation
Primary cell type: - Reprogramming method
Vector type: Integrating Vector: Virus Virus type: Retrovirus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Have the reprogramming vectors been silenced: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: mTeSR Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 21% Temperature: 37C -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles SSEA-1 No POU5F1 (OCT-4) Yes SSEA-4 Yes TRA 1-60 Yes Differentiation potency
Ectoderm: Ectoderm In vitro spontaneous differentiation
Marker Expressed NeuroD Yes PAX6 Yes Endoderm: Endoderm In vitro spontaneous differentiation
Marker Expressed GATA6 Yes TBX5 Yes Mesoderm: Mesoderm In vitro spontaneous differentiation
Marker Expressed AFP Yes SERPINA Yes Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Karyotyping
Passage number: 31 Cell line karyotype: No autosomal or sex chromosome aneupliodies detected Karyotyping method: BOBs Genotyping
Genome-wide analysis: Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase. -
Genetic modification
Genetic modification
Modifications not associated with diseases
Type of modification: Gene knock-in Gene (target): SLC17A7 Transgene: T2A-GFP cassette Chromosome location (transgene): 19q13.33 Delivery method: Zinc finger nuclease Description: Induced pluripotent stem cell line with insertion of T2A-GFP cassette into SLC17a7 gene introduced by zinc finger nuclease (ZFN) technology.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
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