SIGi001-A

IPSC0028 parental, SAMEA4447455

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name SIGi001-A
Alternative name(s)
IPSC0028 parental, SAMEA4447455
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Sigma-Aldrich (SIG)
Owner Sigma-Aldrich (SIG)
Distributors
European Collection of Authenticated Cell Cultures (ECACC)
Sigma-Aldrich (SIG)

External Databases

hPSCreg SIGi001-A
BioSamples SAMEA4447455
Cellosaurus CVCL_EE38
Sigma-Aldrich IPSC0028
CLO CLO_0100807
Wikidata Q54953429

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclones

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 20-24
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Frontotemporal Dementia
In the original line SIGi001-A, no disease was diagnosed. In the line SIGi001-A-14, an HA-tag was added in homozygosity
Synonyms
  • Frontotemporal Dementia
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4447434

hIPSC Derivation#

General

Source cell type
epithelium
Portion of tissue, that consists of one or more layers of epithelial cells connected to each other by cell junctions and which is underlain by a basal lamina. Examples: simple squamous epithelium, glandular cuboidal epithelium, transitional epithelium, myoepithelium[CARO].
Synonyms
  • portion of epithelium
  • epithelial tissue
Age of donor (at collection) 20-24

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768