RCi003-B
Donor information | |
|---|---|
| Gender: | Female |
Disease status | |
| Disease: | pain agnosia |
| Affected: | Yes |
| Disease associated phenotypes: | Chronic Insensitivity to Pain, SCN11a |
General information | |
| Depositor: | Pfizer Limited - Pfizer |
| Cell line name: | RCi003-B |
| Cell line alternative names: | CIP11A-9, RCi117 |
| Biosamples ID: | SAMEA3106143 |
| hPSCreg name: | RCi003-B |
Related lines | |
| From the same donor: | RCi003-A |
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Derivation
Derivation
Primary cell type: Erythroblast Passage number reprogrammed: NA Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: mTeSR Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 21% Temperature: 37C -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles TRA 1-60 Yes SSEA-1 No SSEA-4 Yes POU5F1 (OCT-4) Yes Differentiation potency
Ectoderm: Ectoderm In vitro spontaneous differentiation
Endoderm: Endoderm In vitro spontaneous differentiation
Mesoderm: Mesoderm In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Karyotyping
Passage number: P16 Cell line karyotype: 18 cells out of 20 normal, 1 cell 47,XXX, one cell 45,XX,-17 Karyotyping method: G-Banding Image / Result Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
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