|Nucleotide sequence HGSV:||NM_001040142.1:c.4264A>G|
|Depositor:||Pfizer Limited - Pfizer|
|Cell line name:||PFIZi031-A|
|Cell line alternative names:||B213c1|
Primary cell type: Peripheral blood mononuclear cell
Vector type: Non-integrating Vector: Sendai virus Gene list:
Is the reprogramming vector detectable: No Merthods used: rtpcr
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no
Medium: mTeSR™1 Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 21% Temperature: 37C
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles TRA 1-60 Yes SSEA-1 Yes SSEA-4 Yes POU5F1 (OCT-4) Yes
Ectoderm: Ectoderm In vitro directed differentiation
Marker Expressed HES5 Yes NeuroD1 Yes PAX6 Yes Endoderm: Endoderm In vitro directed differentiation
Marker Expressed CXCR4 Yes GATA6 Yes Mesoderm: Mesoderm In vitro directed differentiation
Marker Expressed MIXL1 Yes NCAM Yes Vimentin Yes Smooth Muscle Cell
Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation
Passage number: 27 Cell line karyotype: 46, XY, modal karyotype in 19/20 cells showed a normal male chromosome and complement banding pattern. One cell 47, XY,+9.
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Cell Line Information Pack
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