PFIZi030-A
Donor information | |
|---|---|
| Gender: | Female |
| Age: | 15-19 |
Disease status | |
| Disease: | Dravet syndrome |
| Affected: | Yes |
| Carrier: | Yes |
| Disease variant | |
| Gene: | NPR2 |
| Chromosome location: | 9p13.3 |
| Nucleotide sequence HGVS: | NM_003995.3:c.890C>T |
| Description: | P.Thr297Met (ACG>ATG) c.890 C>T in exon 3 in the NPR2 gene |
General information | |
| Depositor: | Pfizer Limited - Pfizer |
| Cell line name: | PFIZi030-A |
| Cell line alternative names: | B216c13 |
| Biosamples ID: | SAMEA104492931 |
| hPSCreg name: | PFIZi030-A |
Timepoint: Confluency
Magnification: x4
Timepoint: Confluency
Magnification: x10
-
Derivation
Derivation
Primary cell type: Peripheral blood mononuclear cell Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: No Merthods used: rtpcr Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: mTeSR™1 Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 21% Temperature: 37C -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles SSEA-4 Yes SSEA-1 No TRA 1-60 Yes POU5F1 (OCT-4) Yes Differentiation potency
Ectoderm: Ectoderm In vitro directed differentiation
Marker Expressed HES5 Yes NeuroD1 Yes PAX6 Yes Endoderm: Endoderm In vitro directed differentiation
Marker Expressed CXCR4 Yes GATA6 Yes Mesoderm: Mesoderm In vitro directed differentiation
Marker Expressed MIXL1 Yes NCAM Yes Vimentin Yes Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Karyotyping
Passage number: 14 Cell line karyotype: 46, XX modal karyotype in 20 cells showed a normal female chromosome and complement banding pattern Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
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