PFIZi012-A

FB66c17, RCi198

iPSC line

Stock unknown
At European Collection of Authenticated Cell Cultures (ECACC)
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name PFIZi012-A
Alternative name(s)
FB66c17, RCi198
Cell line type Human induced pluripotent stem cell (hiPSC)
Notes This iPS cell line can only be used for research on Autism Spectrum Disorders or related neurodevelopmental disorders.

Provider

Depositor Pfizer Limited - Pfizer (PFIZ)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg PFIZi012-A
BioSamples SAMEA4097192
ECACC 66540228
Cellosaurus CVCL_VE73
Wikidata Q54947266

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
chromosome 16p11.2 deletion syndrome
The donor is affected.
Synonyms
  • distal 16p11.2 microdeletion syndrome

External Databases (Donor)

BioSamples SAMEA4097181

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX,der(20)t(3;20)(p21;p11.2)
Passage number: P26
Karyotyping method: G-Banding

Other Genotyping (Cell Line)