LUBi001-C
Donor information | |
|---|---|
| Gender: | Male |
| Age: | 65-69 |
Disease status | |
| Disease: | Frontotemporal dementia |
| Affected: | Yes |
| Carrier: | Yes |
| Disease variant | |
| Gene: | GRN |
| Chromosome location: | 17q21.31 |
| Nucleotide sequence HGSV: | NM_002087.3:c.1477C>T |
| Protein sequence HGSV: | NP_002078.1:p.Arg493Ter |
| Description: | Progranulin: R493X mutation |
General information | |
| Depositor: | H. Lundbeck A/S |
| Cell line name: | LUBi001-C |
| Cell line alternative names: | PGRN-8310, RCFB58 c3.7, RCi200, PGRN8310 |
| Biosamples ID: | SAMEA4309665 |
| hPSCreg name: | LUBi001-C |
Related lines | |
| From the same donor: | LUBi001-B |
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Derivation
Derivation
Primary cell type: Fibroblast Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: Essential E8 Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 21% Temperature: 37C -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles TRA 1-60 Yes SSEA-1 No SSEA-4 Yes Differentiation potency
Ectoderm: Ectoderm In vitro spontaneous differentiation
Endoderm: Endoderm In vitro spontaneous differentiation
Mesoderm: Mesoderm In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Karyotyping
Passage number: 18 Cell line karyotype: 46,XY Karyotyping method: G-Banding Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
Cell Line Information Pack
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