LUBi001-A

PGRN-8310, PGRN8310, RCi195, RCFB58 c12.5

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name LUBi001-A
Alternative name(s)
PGRN-8310, PGRN8310, RCi195, RCFB58 c12.5
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor H. Lundbeck A/S (LUB)
Distributors
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg LUBi001-A
BioSamples SAMEA4096428
Cellosaurus CVCL_VE41
CLO CLO_0101592
Wikidata Q54903225

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 65-69

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Frontotemporal dementia
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • FTD
Genetic variants
GRN (target)
17q21.31
NM_002087.3:c.1477C>T
NP_002078.1:p.Arg493Ter
Progranulin: R493X mutation

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4096476

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 65-69

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CXCR4
Unknown
GSC
Unknown
FOXA2
Unknown
GATA6
Unknown
SOX17
Unknown
PITX1
Unknown
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
DCN
Unknown
NCAM1
Unknown
MIXL1
Unknown
VIM
Unknown
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
NeuroD1
Unknown
PAX6
Unknown
HES5
Unknown

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: P22
Karyotyping method: G-Banding

Other Genotyping (Cell Line)