ESi003-A

[CRTRd]FiPS3819-4F-2

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name ESi003-A
Alternative name(s)
[CRTRd]FiPS3819-4F-2
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Spanish Stem Cell Bank (ES)
Owner Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB)
Distributors
Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB)
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Spain

External Databases

hPSCreg ESi003-A
BioSamples SAMEA3303029
Cellosaurus CVCL_V154
ECACC 66540064
CLO CLO_0101545
Wikidata Q54832762

General Information

* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
X-linked creatine transporter deficiency
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Creatine transporter deficiency
  • SLC6A8 deficiency
Genetic variants
SLC6A8 (target)
Xq28
NM_005629.3:c.1396G>A
Disease associated phenotypes
  • CRTR deficiency 1396 G>A

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

External Databases (Donor)

BioSamples SAMEA3303031

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphological
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Gelatin
Feeder cells Human foreskin fibroblasts
Cellfinder Ont Id: CELDA_000001419
Passage method Mechanically
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout Dulbecco's Modified Eagle's medium
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
Glutamax 2 mM
2-Mercaptoethanol 0,05 mM
bFGF 8 ng/ml
NEAA 1 %
Penicillin-Streptomycin 0,5 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
GATA4
Yes
AAS
Yes
ASMA
Yes
ASA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
Tuj1
Yes
GFAP
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 19
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes