EPFLi002-A

hiPS9

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name EPFLi002-A
Alternative name(s)
hiPS9
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Swiss Federal Institute of Technology (EPFL)
Distributors
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg EPFLi002-A
BioSamples SAMEA104012164
Cellosaurus CVCL_VD35
CLO CLO_0100650
Wikidata Q54832357

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA104012325

hIPSC Derivation#

General

Source cell type (free text) CD34+ Hematopoietic stem cell

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
ZFP42 (REX-1)
Yes
LIN28A
Yes
DPPA2
Yes
Marker Present Absent
mCpG
OCT4
Transcriptome Characterisation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)