EDi018-A

RCi211

iPSC line

The cell line has been recalled.

General#

Cell Line

hPSCreg Name EDi018-A
Alternative name(s)
RCi211
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University of Edinburgh (ED)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg EDi018-A
BioSamples SAMEA4771918
Cellosaurus CVCL_LE79
Wikidata Q54832007

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
bipolar disorder
Bipolar Disorder Type II
Synonyms
  • Psychoses, Manic-Depressive
  • Bipolar affective disorder, current episode depression (disorder)
  • Manic bipolar I disorder
  • Manic-depressive psychosis
  • mixed bipolar affective disorder, NOS (disorder)
  • Disorder, Bipolar
  • Manic bipolar I disorder (disorder)
  • Manias
  • Bipolar Disorders
  • Affective Bipolar Psychosis
  • Psychosis, Bipolar Affective
  • Psychosis, Manic-Depressive
  • Manic Depressive Psychosis
  • MANIC DIS
  • Bipolar Depression
  • BIPOLAR DIS
  • Manic Disorders
  • Unspecified bipolar affective disorder, NOS (disorder)
  • Bipolar affective disorder
  • Manic States
  • Unspecified bipolar affective disorder
  • State, Manic
  • Psychoses, Manic Depressive
  • MANIC DEPRESSIVE ILLNESS
  • Mania
  • bipolar disorder manic phase
  • Unspecified bipolar affective disorder, NOS
  • Psychoses, Bipolar Affective
  • Unspecified bipolar affective disorder, unspecified (disorder)
  • Unspecified bipolar affective disorder, unspecified
  • Psychosis, Manic Depressive
  • Bipolar affective disorder , current episode mixed (disorder)
  • Disorder, Manic
  • Manic-Depressive Psychoses
  • Manic Disorder
  • States, Manic
  • mixed bipolar disorder
  • [X]Bipolar affective disorder, unspecified (disorder)
  • Unspecified bipolar affective disorder (disorder)
  • Bipolar disorder, unspecified
  • Bipolar Affective Psychosis
  • Manic-depressive syndrome NOS
  • Manic Bipolar Affective disorder
  • Manic State
  • Bipolar affective disorder, manic, unspecified degree
  • Bipolar disorder (disorder)
  • mixed bipolar affective disorder (disorder)
  • Affective Psychosis, Bipolar
  • [X]Bipolar affective disorder, unspecified
  • bipolar disease
  • Bipolar affective disorder, mixed, unspecified degree
  • MDI - Manic-depressive illness
  • Manic-depressive illness
  • Bipolar disorder, NOS
  • BIPOLAR DISORDER NOS
  • mixed bipolar I disorder (disorder)
  • Depression, Bipolar
  • Depressive-manic psych.
  • Manic-Depression
  • manic-depression
  • major affective disorder
  • bipolar depression
  • bipolar disorder
  • Manic Depressive disorder
show more synonyms
Family history From multiple affected family multiple affected by bipolar and major massive depressive disorder

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4772668

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No autosomal or sex chromosome aneuploidies were detected
Passage number: 18
Karyotyping method: BACS-on-Beads

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768